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Fig. 1. pixie is an enhancer of a KD-Dp110-induced small-wing phenotype and shows Minute-like characteristics. (A-D) MS1096-Gal4 driven expression of UAS-KD-Dp110 reduces wing size and this phenotype is enhanced by pix3c2/+ and pix3c3/+. The genotype MS1096>KD-Dp110 is abbreviated to KD-Dp110. (E) Bar chart showing the average areas of the wings in A-D, n=8-12. (F,G) pix3c2/+ bristles (G) are shorter and more slender than control (F). (H-K) pixie mutant and M(3)66D/+ wings. Patterning defects are absent. Controls match the genetic background of the mutations, CS, CantonS. (L,M) Bar charts showing average ratio of wing/thorax circumference (L) and wing area (M) for the genotypes in H-K, n indicates numbers of flies/wings analyzed. When compared with control, the ratio in L is higher in pixL17/L35 (pix) flies and M66D1/+ (M) flies (P=3.4x10-10 and 0.0008, respectively). pixie mutant wing area is not significantly different from control, whereas M66D1/+ wings are significantly larger than control (P=0.15 and 3.7x10-25, respectively).





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