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Fig. 4. pixie mutant and Minute wing imaginal discs show
regionally elevated levels of apoptosis. (A-L) Images are projections of
confocal z-series taken through TUNEL-labelled wing imaginal discs
that have been then inverted to reveal wing disc morphology, disc genotypes
are indicated. Discs from (A,B) early to mid-third instar, (F,H) late third
instar and (C-E,G,I-L) wandering larvae. Apoptosis is similar to control in
discs heterozygous for pixL17 (D) and
pixL35 (E), and is enhanced in discs that are
transheterozygous for pixL17 and
M(3)66D1 (J) and pixL17 and
M(3)95A1 (L). Thin arrow in G indicates apoptotic nuclei
that lie approximately at the DV boundary; thick arrow indicates those that
lie at the pouch borders. (M) Bar charts and wing images showing that
expressing p35 in the posterior compartment increases area in
pixL17/L35 wings (pix) compared with control
(pixL17/+). P/A ratio in pix wings is
significantly higher than control, P=3.8x10-10.
White line in images on the right indicates the AP boundary. (N) Wg protein,
detected by immunostaining, is ectopically expressed (arrow) when p35 is
expressed in the posterior compartment of pixie mutant discs. Scale
bar: 50 µm in A-L.
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