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Fig. 5. pix^L17 causes a reduction in balanced growth and cell survival in wing discs. (A) A pix^L17, f^36a wing clone (red) induced 27 hours before larval wandering and its accompanying mwh twin clone (blue). The presence of the mutant clone does not affect the arrangement of wing hairs in rows, indicating that mutant cell size is not altered. Bar chart on the right shows frequency and cell number of pix^L17 wing clones (black bars) compared with their twins (grey bars). (B) GFP^-, pix^L17 clones and their GFP^+/+ twins (examined at wandering stage and induced 46 hours before) in wing discs expressing the capsase inhibitor p35 in the posterior compartment (labelled using anti-En, red or orange when merged with GFP). (C) Apical (above) and basal (below) confocal sections through the same disc showing that nuclei of surviving pix^L17 clones (white arrow) in the posterior pouch are more basal than their accompanying twins (black arrow). (D) Bar charts representing the number of cells in pix^L17clones (black), and their twins (grey) in anterior clones (above) and in posterior p35-expressing clones (below); pouch clones are on left and hinge clones on right. TUNEL labelling confirmed that p35 expression blocked apoptosis (data not shown). Although posterior mutant clones are larger, the average mutant/twin clone size in the posterior compartment is still significantly less in the pouch (0.17) than the hinge (0.33), P<0.0001.

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