|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Examination of cilia in the Wnt3a-/- mutants. (A,B)
High-power SEM micrographs of cilia in wild-type (A) and
Wnt3a-/- (B) nodes of head-fold stage embryos
(
E7.75-8). (C,D) Projection images of node cilia visualized using
anti-acetylated tubulin antibodies. (E) Two-color whole-mount in situ
hybridization analysis of Lefty1 (orange) and Lrd (purple)
expression in three-somite wild-type (left) and Wnt3a-/-
(right) embryos. (F) Wnt3a (orange) and Nodal (purple)
expression in three iv/iv embryos. (G-Q) Confocal microscopy images of PC1
(G,K), PC2 (H,L), acetylated tubulin (I,M) and the merged images (J,N,O,P,Q)
in E7.75 wild-type (G-J,O) and Wnt3a-/- (K-N,P,Q) nodes.
The abundance of white spots in global views of the node indicated
co-expression of all three markers in individual wild-type cilium (arrows,
G-J). By contrast, Wnt3a-/- cilia predominantly appeared
as purple spots (N), indicating co-expression of only PC2 and tubulin. Views
were selected to present sufficient numbers of labeled cilia and therefore
depict slightly different regions of the wild-type and mutant node; however,
images are representative of the entire node. Profiles of labeled cilia
illustrate the co-expression of PC1 and PC2 in distinct spatial domains in
wild-type cilia (arrows, O). Rare mutant cilia co-expressed PC1 and PC2 but
these domains were unusually small and not easily detected (arrow, P). Cilia
co-expressing PC2 and tubulin were easily detected in the mutant node (Q).
|
