DEV02095 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Confocal images, indicating expression of GFP though a 188.5 mm depth of injection/electroporation organ cultures. Successive 6.5 mm sections are shown. (A) Empty vector injection. (B) Gfp vector injection.

• Supplemental Figure 2 -

  Fig. S2. Effect of electroporation on branching morphogenesis and gene expression, as observed by immunostaining for organ cultures after growing 48 hours. (A,B) Co-staining of cytokeratin (red) and Pax2 (green). (C,D) Co-staining of cytokeratin (red) and Wt1 (green). (A,C) No electroporation. (B,D) Electroporation. (A,B) and (C,D) Same embryo. No significant differences in Pax2 or Wt1 expression were shown between organ cultures with or without electroporation. (E) Quantitation of eight pairs of organ cultures represented by A,B or C,D, indicating small differences in branching morphogenesis.