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Fig. 2. Mapping of the lsy-2 locus. (Top) A genetic map (not drawn to scale); (bottom) a physical map. lsy-2 was mapped with the SNP markers pkP6142 and pkP6144 in the Hawaiian C. elegans isolate CB4856 (Hodgkin and Doniach, 1997; Wicks et al., 2001). An unc-1 lsy-2 dpy-3 triple mutant was generated and used to three-factor map lsy-2 between pkP6143 (located on cosmid F49H12) and dpy-3 (EGAP7.1). The blue dots in the schematic presentation of the LSY-2 protein (not drawn to scale) indicate Cys- and Hischelated zinc atoms; the numbers indicate the spacing between individual Cys and His residues. NLS, putative nuclear localization sequence.





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