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Fig. 4. lsy-2 is expressed ubiquitously and localizes to moving speckles in the nucleus. (A) lsy-2^prom::gfp and lsy-2::gfp reporter gene constructs used in this study. The gray bar behind the green gfp sequence indicates the heterologous unc-54 3'UTR. (B) lsy-2^prom::gfp and lsy-2::gfp reporter gene constructs are ubiquitously expressed at different developmental stages. Nine independent lsy-2::gfp transgenic lines and three independent lsy-2^prom::gfp lines show similar expression patterns. (C) lsy-2::gfp is expressed in ASEL and ASER. ASEL and ASER are labeled with the ceh-36^prom::rfp transgene otIs151. Note that the rfp reporter is diffusely localized throughout the cytoplasm, whereas the gfp signal is in nuclear speckles. lsy-2::gfp rescues the lsy-2 mutant phenotype (Table 3). (D) lsy-2::gfp is localized to moving nuclear speckles. Individual time frames of a movie, shot with Openlab Software at a time interval of half a second per frame (20 frames total), are displayed. The movie is available upon request.

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