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Fig. 1. Ptf1a is in precursors to multiple neural tissues and overlaps with Mash1 in the dorsal neural tube. The Ptf1a lineage was visualized by ß-gal staining of Ptf1a^Cre/+;R26R-stop-lacZ^+/- mice. (A) E11.5 whole mount, showing ß-gal staining in the dorsal neural tube (inset) from the mid-hindbrain to the tail (arrowheads). Staining in the pancreas is indicated by the arrow. (B) brain and spinal cord dissected from an E16.5 embryo, stained for ß-gal, showing expression in cerebellum (arrow) and dorsal spinal cord (arrowhead). Ventral diencephalon also reveals cells from the Ptf1a lineage (inset). (C,D) Adult cervical spinal cord, showing ß-gal stained cells primarily in laminae I-IV. The boxed area in C is shown at higher magnification in D. Arrows (C) highlight labeled cells found ventral to boxed area. (E) Adult cerebellum with ß-gal-stained Purkinje, stellate, basket and Golgi cells. (F) Coronal section of the adult brain reveals ß-gal-stained cells in the ventral hypothalamus. Tissue is counterstained with Nuclear Fast Red (C,D,F) and Nissl (E). Immunofluorescence on transverse neural tube sections of E10.5 (G,H) and E11.5 (I,J) wild-type mice. (G,I) Ptf1a (red), Mash1 (green) and their co-labeling (yellow) in a subset of cells. (H,J) Ptf1a (red) and BrdU incorporation (green) are largely non-overlapping. The asterisk in H indicates a rare BrdU-incorporating Ptf1a cell. Scale bar: 1 mm in A; 50 µm in D-J; 12.5 µm in the insets. ba, basket cells; dI3-dI5, dorsal interneurons 3-5; Go, Golgi cells; MZ, marginal zone; Pu, Purkinje cells; st, stellate cells; VZ, ventricular zone.

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