QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. dI4 are trans-fated to dI5 interneurons in Ptf1a-deficient embryos. Immunofluorescence on neural tube transverse sections of Ptf1a^Cre/+ (A,C) and Ptf1a^cre/cre (B,D) mouse E10.5 embryos. (A,B) dI4 neurons marked in yellow by co-labeling of Lhx1/5 (red) and Pax2 (green) are lost in the Ptf1a null (arrows). (C,D) Lmx1b (red) labeling dI5 is expanded in Ptf1a null embryos, while Isl1 (green) labeling dI3 are not affected. (E-H) Anti-GFP antibody was used to detect YFP in E10.5 Ptf1a^Cre;R26R-stop-YFP embryos. YFP acts as a lineage marker for cells that have expressed the Ptf1a locus. YFP (green) co-localizes with the Lhx1/5 (dI4;red), but fails to do so in Ptf1 null embryos (compare E and F). (G) Lmx1b (dI5;red) does not co-localize with YFP in the presence of Ptf1a, but they do co-localize in the null (H). The ventricle is to the left in all panels, as shown in a schematic representation of the markers used to distinguish individual neuronal populations (I). (J) Cell counts for each neuronal population. (K) Summary diagram showing that Ptf1a is required for dI4 and suppression of dI5 interneurons (see Results). Scale bar: 50 µm in A-D; 25 µm in E-H.

Return to article