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Fig. 3. dIL^A trans-fate to dIL^B interneurons in Ptf1a null embryos. Immunofluorescence on neural tube transverse sections of Ptf1a^Cre/+ (A,C) and Ptf1a^Cre/Cre (B,D) mouse E12.5 embryos. (A,B) dIL^A neurons, marked by Lhx1/5 (red) and Pax2 (green), are lost specifically in the dorsal neural tube, but not the ventral neural tube in the Ptf1a null (dashed line indicates the position of the ventricle). (C,D) dIL^B neurons, marked by Lmx1b (red) increase in the Ptf1a null, but there is no change in the number of Isl1 (green) cells. (E-J) Anti-GFP antibody was used to detect YFP at E12.5 in Ptf1a^Cre;R26R-stop-YFP embryos. YFP acts as a lineage marker for cells that have expressed the Ptf1a locus. (E-F') In the presence of Ptf1a, YFP (green) co-localizes (yellow) with the dIL^A marker, Lhx1/5 (red), but it does not do so in Ptf1a null embryos. (G-H') Lmx1b (dIL^B; red) and (I-J) Tlx3 (dIL^B; red) rarely co-localizes with YFP in embryos with Ptf1a, while substantially more Lmx1b/YFP and Tlx3/YFP double positive cells appear in Ptf1a-deficient embryos (yellow). The boxed regions in E-H are shown at higher magnification in E'-H'. (K) Cell counts of specific neuronal populations. (L) Summary diagram showing that Ptf1a is required for the dIL^A interneuron cell fate, while suppressing dIL^B. Scale bar: 50 µm in A-H; 25 µm in E'-H',I,J.

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