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Fig. 5. otx2, fgf8 and six4.1 genes are expressed in a normal
spatio-temporal pattern in the foxd3+sox10 morphant embryos.
(A-D) Uninjected embryos showing the pattern of otx2 (A,B),
fgf8 (C) and six 4.1 (D) gene expression at 35±1 hpf.
(E-H) Corresponding pattern of gene expression of otx2 (E,F),
fgf8 (G) and six 4.1 (H) in foxd3+sox10
morphant embryos at 35±1 hpf. (A,E) Lateral views with MHB gene
expression (arrow), and pigment cells in the uninjected animals (A,
arrowhead). (B,F) Dorsal view showing otx2 expression in the
midbrain; asterisks indicate the approximate location of the gnrh2
cells at this stage of development. (C,G) Dorsal views showing that
fgf8 expression (arrows) is maintained at the MHB. (D,H) Ventral view
of olfactory organs (arrows) expressing six4.1; asterisks indicate
where the gnrh3 cells would be found at this stage of development.
Scale bars: 100 µm in A,E; 50 µm in B,C,F,G; 100 µm in D,H.
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