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Fig. 7. BMPs regulate the maturation of GFAP-expressing cells in vivo. (A-H) GFAP (green), and SOX1 or vimentin (red) in the hippocampus SGZ (A-C,G) and ML (D-F,H) in P15 wild-type (B,E), NSE-noggin (A,D,G,H) and NSE-BMP4 (C,F) animals. Cells were counterstained with Hoechst (blue). (I,J) Number of SOX1-(I) and vimentin-(J) expressing GFAP⁺ cells. (A,B,D,I,J) GFAP-expressing cells in the SGZ remain as progenitors and increase in number when noggin is overexpressed, as assessed by SOX1 and vimentin co-labeling. (C,I,J) Overexpression of BMP4 in the SGZ promotes the loss of progenitor markers in GFAP⁺ cells. (D) Confocal image demonstrating co-localization of SOX1 and GFAP in the SGZ of noggin mice. (F,G,I,J) GFAP-expressing cells in the ML rarely co-express SOX1 or vimentin in wild-type or overexpressed BMP4 mice. (E,I,J) BMP inhibition in the ML increases progenitor markers in GFAP⁺ cells. (H) Confocal image demonstrating co-localization of SOX1 and GFAP in the ML of noggin mice. (I) ^*P<0.02, ^**P<0.05; (J) ^*P<0.03, ^**P<0.01 ANOVA. Scale bar: in C, 10 µm for A-C,G,H; in E, 20 µm for D-F.

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