DEV02159 Supplementary Material

Files in this Data Supplement:

• Supplemental Table - Adobe PDF

• Supplemental Figure 1 -

  Fig. S1. sqd^j4B4 germline clones display previously characterized sqd phenotypes. (A,B) Expression of the marker mirror^6D1 (blue) is confined to the dorsal follicle cells in wild-type egg chambers (A) but encircles the whole anterior circumference of the oocyte in sqd^j4B4 germline clones (B). (C,D) Grk protein (red) localizes to the dorsal anterior corner of wild-type stage 8-10 oocytes (C) but expands along the entire anterior cortex of sqd^j4B4 germline clone oocytes (D). (E,F) Propidium iodide staining in wild-type egg chambers reveals the nurse cell nuclei to be condensed in a ‘blob-like’ appearance in early egg chambers (arrow), whereas in stage 7-8 egg chambers, the nurse cell nuclei decondense and take on a more ‘puffy’ appearance (E). In sqd^j4B4 germline clones, the nurse cell nuclei remain condensed and ‘blob-like’ even in later stages (F). (G,H) Sqd protein (green) is detectable in the nurse cells, oocyte nucleus (arrowhead), oocyte cytoplasm (arrow) and follicle cells of wild-type egg chambers (G), as previously reported (Matunis et al., 1994), but it is absent from the germline of sqd^j4B4 germline clones (H).

• Supplemental Figure 2 -

  Fig. S2. sqd mutants have defects in egg chamber morphology and follicle cell migration. (A,B) Orb protein (green) marks a single oocyte at the posterior of the egg chamber in wild-type ovarioles (A); in sqd^j4B4 germline clones, egg chambers sometimes contain no oocyte (B). Rhodamine-phalloidin (red) labeling of actin reveals the cell outlines and the absence of stalk cells between sqd mutant egg chambers (B). (C) Propidium iodide staining of sqd^j4B4 germline clones reveals egg chambers with aberrant germ cell numbers. Arrow points to an egg chamber with two nurse cells and no oocyte. (D) sqd^j4B4 mutant egg chambers sometimes have an incorrectly positioned oocyte, as revealed here by Dynein heavy chain staining (green, arrow) and propidium iodide (red). Arrowhead points to an egg chamber with the oocyte at the correct position at the posterior. (E,F) a-tubulin staining (green) of wild-type stage 10 egg chambers shows border cells at the anterior of the oocyte (E, arrow); a majority of stage 10 sqd^j4B4 germline clone egg chambers have delayed border cell migration (F, arrow).