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Fig. 2. sqd mutants display AP patterning defects at mid-oogenesis. (A,C,E) In sqd^j4B4 germline clones, kin-ß-gal (A, blue), GFP-Stau (C, green) and osk RNA (E, purple) are mislocalized to an ectopic spot in the center of the oocyte at almost complete penetrance. (B,D,F) Weaker mislocalization phenotypes are observed for kin-ß-gal (B, blue), GFP-Stau (D, green) and osk RNA (F, purple) in sqd^j4B4/sqd¹ (B,F) and sqd¹/sqd¹ (D) egg chambers. Arrows point to ectopic GFP-Stau (C,D) and osk RNA (E,F). Rhodamine-phalloidin (red) marks cell outlines in C and D. (G,H) Dynein heavy chain (green) localizes to the posterior in wild-type stage 9-10 oocytes (G) but not in sqd^j4B4 germline clone egg chambers (H). (I,J) GFP-Stau (green) appears as diffuse particles in the nurse cell cytoplasm of wild-type egg chambers (I), while in sqd^j4B4 germline clones, it aggregates around the nurse cell nuclei (J). Nuclei are labeled with propidium iodide (red). Note that sqd^j4B4 germline clone oocytes are often smaller than wild-type oocytes in mid- to late oogenesis.

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