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Fig. 6. Abnormal targeting of mammillary body axons as revealed by DiI labelling. Crystals of DiI were inserted into E14.5 brains of Sim1+/-;Sim2+/- (A,B) and Sim1-/-;Sim2-/- (C,D) embryos, and the brains sectioned after an incubation period of 2 weeks. (B,D) Higher magnification images of A (B) and C (D). The white line in B and D corresponds to the midline. The PMT (arrowhead) is recognizable in Sim1+/-;Sim2+/- but not in the double mutant. A few axons appear to progress towards the midline in Sim1+/-;Sim2+/- embryos (arrow), whereas the majority of them do so in Sim1-/-;Sim2-/- embryos.





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