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Fig. 1. Ferlin protein expression during muscle development. (A) Myoferlin and
dysferlin are 230 kDa transmembrane proteins in the ferlin family. Their amino
acid sequences are 74% similar and contain six cytoplasmic C2 domains.
Myoferlin and dysferlin are expressed in skeletal muscle and in the muscle
cell line C2C12. (B) During C2C12 cell differentiation, immunoblotting showed
that myoferlin was expressed earlier in differentiation than was dysferlin.
The asterisk indicates the switch from growth to differentiation media. These
timepoints represent cultures containing a mixture of myoblasts and myotubes.
(C) Images taken during C2C12 differentiation, corresponding to the timepoints
shown in B. Scale bar: 50 µm. The same cell cultures were co-stained for
myoferlin (red) and dysferlin (green). Myoferlin was expressed earlier and was
expressed highly in singly nucleated myoblasts. Dysferlin expression was
detected only in multinucleated myotubes.
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