|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Proliferating cells in P8 control and mutant brains express progenitor cell
markers, not a mature astrocyte marker. Sections from P8 control and
Nf1hGFAPKO mutant brains were subjected to double-labeling
immunofluorescence with anti-Ki67 (red) and anti-nestin (green) (A,B), with
anti-BrdU (red) and anti-BLBP (green) (C,D), and with anti-BrdU (red) and
anti-GFAP (green) (E,F). BrdU staining in the external granule cells in the P8
cerebella serves an internal positive control for this study (E,F). (G,H)
Higher magnification of sections in E,F, showing that only a small number of
BrdU-positive cells in the mutant brain but not in the control brain expressed
GFAP (arrows in H). (A-D) Sections from the thalamus. (E-F) Sections from the
brainstem. IV, the fourth ventricle. Scale bars: 100 µm.
|
