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Fig. 4. Nf1^-/- glial progenitor cells undergo astrocytic differentiation. (A) Sections from P8 control (a,c,e) and Nf1^hGFAPKO mutant (b,d,f) brains were subjected to double-labeling immunofluorescence with anti-Cre (red) and anti-GFAP (green). (a,b) Sections from the control and mutant cerebral cortex (CC); (c-f) low (c,d) and high (e,f) magnification of sections from control and mutant hippocampal dentate gyrus (DG). Most of the GFAP-positive cells also express the Cre transgene. (B) Sections from P8 control (a,c,e) and Nf1^hGFAPKO mutant (b,d,f) brains were subjected to double-labeling immunofluorescence with anti-nestin (red) and anti-GFAP (green). (a,b) Sections from the corpus callosum; (c,d) sections from the brainstem; (e,f) sections from the dentate gyrus. Most of the GFAP-positive astrocytes in both control and mutant P8 brains do not express nestin, except in the dentate gyrus (arrows in e,f), where a small number of cells express both GFAP and nestin. There are low levels of nestin expression in the endothelial cells. Genotypes: control mice, Nf1^flox/+;hGFAP-cre+; mutant mice, Nf1^flox/-;hGFAP-cre+ or Nf1^flox/flox;hGFAP-cre+. Scale bars: 100 µm.

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