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Fig. 9. Nf1^hGFAPKO hyperplastic nerves have increased proliferation and express glial progenitor cell markers. (A) The quantification of BrdU-positive cells in the adult control (CTR) and mutant (CKO) distal (DN) and proximal (PN) nerves. The data plotted are mean±s.e.m. (distal nerve, CTR, n=4, CKO, n=5, ^**P<0.001; proximal nerve, CTR, n=3, CKO, n=6, ^**P<0.001). Sections from control (B,D) and mutant (C,E) distal (B,C) and proximal (D,E) optic nerves were stained with anti-BrdU (red) and anti-GFAP (green). Sections from control (F) and mutant (G) nerves were stained with anti-Ki67 (red) and anti-nestin (green). Arrows in B and C indicate proliferating cells that do not express GFAP. Arrows in F and G indicate nestin-positive proliferating cells (inset in G provides higher magnification). Sections from control proximal (H,J), mutant proximal (I,K), control distal (L) and mutant distal (M) optic nerves were stained with anti-BLBP. Arrow in J indicates a BLBP-positive cells with normal nuclei in the normal nerve; arrows in K,M indicate BLBP-positive cells with atypical nuclei in the mutant nerve. Sections from control (N) and mutant (O) optic nerves were stained with anti-Pax2. Scale bars: 100 µm.

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