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Fig. 4. Western analysis of Vls. Ponceau Red staining serves as a loading control and is shown below the corresponding blot. (A) Anti-Vls antibodies detect a protein of $~$ 42 kDa in wild-type ovaries and in ovaries expressing one copy of the CG10728⁺/vls⁺ rescue construct. This antigen is not detected in vls^null mutants, nor in vls^EMS hemizygotes and trans-heterozygotes. Genotypes: vls^null - Df(2L)be408 / Df(2L)pr2b, P[w⁺ barr⁺]; vls^null vls⁺ - Df(2Lbe408, P[w⁺ CG10728⁺/vls⁺] / Df(2L)pr2b, P[w⁺ barr⁺]; and Df - Df(2L)be408. (B) Developmental expression profile of Vls. vls^null ovary extracts serve as a control for the specificity of the ${alpha}$ -Vls antibodies. Other protein extracts were collected from wild-type flies. The faint low molecular weight band does not appear to originate from ovaries or embryos.

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