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Fig. 1. Rarb is expressed more anteriorly than Hoxb4 in the
presegmented hindbrain. The anterior neural expression borders of Hoxb4
protein (A,C,E) and Rarb mRNA (B,D,F) are compared at E8.5 and E9.5.
(A,B) At E9.5, neural expression of both genes extends up to a straight border
at the r6/r7 boundary (arrowhead), lying just caudal to the otic vesicle.
(C,D) At E8.5, using the otic sulcus (Os) as a landmark for presumptive r5/r6,
it can be seen that neural Rarb expression extends more anteriorly
than that of Hoxb4. (E,F) At E8.5, with Krox20 expression marking
presumptive r5, it can be observed that Rarb is expressed in all of
presumptive r6 but Hoxb4 is not. At this stage, the anterior expression border
of both genes is diffuse and, for Rarb, weak expression may also
extend into the posterior part of presumptive r5. Anterior is towards the top
in this and all subsequent figures.
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