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Fig. 2. Expression of GFP in the chick inner ear after electroporation of control
IRES-GFP plasmid DNA. (A) Low magnification view of the cochlear duct of an E9
chick embryo electroporated at stage HH 17 (E2.5). In the basilar papilla and
the lagenar macula, each red dot corresponds to the stereociliary bundle of
one hair cell. (B) High magnification view of a more proximal region of the BP
of the same specimen. Scattered GFP-positive cells (green) include both hair
cells (with rounded GFP-positive cell body and HCA-positive apical surface,
arrowheads) and supporting cells (elongated cell bodies and absence of HCA
staining). (C,D) Utricular macula of an E8 chick embryo electroporated at
stage HH 13 (E2). Several groups of GFP-positive cells are present within this
sensory patch. (D) Transverse view of this epithelium at higher magnification.
Some of the GFP-positive cells are hair cells (asterisk), with HCA-positive
hair bundles and HCS-1-positive cytoplasm; others are supporting cells
(arrows), with elongated cell bodies extending below the hair cell layer. All
images other than (D) are projections of confocal optical sections of
immunostained whole mounts; (D) is a single optical section. Counterstain for
actin (blue) in B and C, combined with the red HCA fluorescence, makes hair
bundles appear purple. BP, basilar papilla; L, lagenar macula.
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