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Fig. 7. In-situ hybridisation analysis of GFP mRNA persistence after
electroporation of chick inner ear at stage HH 13 (E2) with
HA-NICD-IRES-GFP construct. Representative micrographs of
whole-mount specimens analysed at 24 hours, 3 days and 7 days
post-electroporation. The number of cells expressing GFP mRNA decreases over
time, and by 7 days post-electroporation scarcely any such cells are visible.
The two cells positive for GFP mRNA in the 7-day specimen (shown enlarged in
the insert) might be ones in which the plasmid has survived by integration
into the host cell genome.
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