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Fig. 2. CNTF/LIF/gp130 receptor signaling does not promote spinal cord neural stem cell (NSC) self-renewal/expansion in vitro. FGFR2 (A) was robustly expressed in the E11.5 spinal cord VZ. By contrast, the expression of CNTFR{alpha} (B) was largely restricted to the mantle zone, demonstrated by ß-tubulin III staining (C). By E14.5, the FGFR2-expressing VZ precursor population (D) was reduced. CNTFR{alpha} (E) continued to be expressed in the mantle zone, indicated by ß-tubulin III (F), though low levels were present in the VZ. (G) CNTF- (**P=0.003) or LIF- (**P=0.007) treated E14.5 spinal cord neurospheres generated significantly fewer secondary neurospheres compared with FGF2 alone (results are normalized to FGF2 control; n=4). (H) CNTF (**P=0.007) or LIF (**P=0.0002) treatment significantly increased the number of ß-tubulin III neurons generated by E14.5 spinal cord NSCs (n=4). (I) Neither CNTF nor LIF significantly increased the number of ß-tubulin III-expressing neurons generated by ventral forebrain NSCs. Scale bars: in A, 50 µm for A-C; in D, 100 µm for D-F.





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