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Fig. 3. Gross morphological changes in the forebrains of E12.5
Lifr-/- embryos relative to control littermates are
suggestive of defects related to growth. At rostral regions of the forebrain,
the LGE (arrow) in Lifr-/- embryos (B) was smaller
relative to Lifr+/+/Lifr+/- (A)
littermates. At more caudal regions, decreased thickness of the cerebral wall
was observed (arrowhead), as well as decreased size of the LGE and MGE
(arrows) in Lifr-/- (D,F) relative to
Lifr+/+/Lifr+/- embryos (C,D). At the
most caudal regions analyzed (G,H), a reduced caudal ganglionic eminence
(arrow) was clearly observed (56% penetrant; Lifr-/-n=9; Lifr+/+/Lifr+/-
n=9). Measurements of the area of the LGE revealed a 22% decrease in
size in Lifr-/- embryos (I,J; paired t-test
**P=0.008; n=5). A concurrent decrease in the
mantle zone was also observed, as shown by ß-tubulin III staining (K,L).
The number of dying cells in the LGE of Lifr-/- embryos
indicated by TUNEL labeling was normalized to the area of the LGE (M;
0.5±0.1 cells/unit area) and no difference was observed relative to
Lifr+/+/Lifr+/- embryos (N;
0.5±0.1 cells/unit area; n=4; arrows indicate positive cells;
stars indicate examples of autoflorescence). The number of BrdU+ precursors in
Lifr+/+/Lifr+/- (O;
865±41) was significantly higher than Lifr-/-
embryos (P; 608±15; paired t-test
**P=0.009; n=5). Scale bar: in A, 100 µm for
A-H; in I, 100 µm for I,J; in K, 50 µm for K,L; in M, 50 µm for
M-P.
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