spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Spatiotemporal expression pattern of XPACE4. (A) Real-time RT-PCR analysis of the wild-type embryos at the indicated stages shows that XPACE4 mRNA is abundant maternally and its level increases pre-MBT (stage 6 and 8) and gradually declines after gastrulation (stage 10 and 12.5). (B) The comparison of oligo dT (dT) versus random hexamer (R6) primed cDNA shows the increase in XPACE4 levels pre-MBT is due to polyadenylation of the maternal mRNA. (C) Whole-mount in situ hybridization shows that XPACE4 is localized during early oogenesis. Inset shows XPACE4 mRNA localization in the mitochondrial cloud of stage 1 oocytes. No signal is detected with the sense probe (S). (D) XPACE4 is localized to the vegetal hemisphere of full-grown oocytes. (E,F) Hemisected stage 10 (E) and stage 11 (F) embryos show XPACE4 mRNA remains localized to the endodermal precursors during gastrulation (dorsal side on the right). (G) At stage 30, XPACE4 is detected in a group of cells in the endoderm after the embryos are bleached and cleared. (H) At stage 35/36, XPACE4 is detected in the olfactory bulb, the brain and the notochord. The lower embryo is the sense control. Inset shows the notochord in transverse sections. S, sense control.





Right arrow Return to article