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Fig. 2. Phenotype of mutants with defects in embryo sac development. (A) Mature wild-type embryo sac. (B) Arrested embryo sac containing a functional megaspore and degenerating spores (EDA 8). (C) Mutant presenting an embryo sac arrested at the two-nuclear stage (EDA 1). The vacuole is indicated and the arrowheads indicate the nuclei. (D) Mutant with an embryo sac arrested at the four-nuclear stage (EDA 4). The vacuole is indicated and the arrowheads indicate the nuclei. (E) Embryo sac showing abnormal number and position of nuclei (EDA 13). The arrowheads indicate the nuclei positions. (F) Polar nuclei fail to fuse (EDA 27). Ant, antipodal; Syn, synergide; Ec, egg cell; Ccn, central cell nucleus; Vac, vacuole; Fm, functional megaspore; Pn, polar nuclei. Scale bar: 25 µm.





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