DEV01591 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Orientation of hair bundles in individual trunk neuromasts. Larvae were fixed and labeled with Alexa488-Phalloidin. Left column, neuromasts of wild-type siblings. Right column, neuromasts of mutant orbiter^th263b larvae. Round shadows represent unstained kinocilia. Two orientations of the kinocilia along the anteroposterior axis are visible. The arrangement of these opposing groups of hair bundles is variable between neuromasts. The axis of orientation does not differ between wild-type siblings and mutant orbiter larvae. Scale bar: 1 mm.

• Supplemental Figure 2 -

  Fig. S2. Injection of pcdh15b morpholinos (40 ng) does not affect overall morphology of larvae (4 dpf). (A) Uninjected larvae. Larvae injected with a 4 bp mismatch control (B), the pcdh15b GT (splice site) (C) or pcdh15b ATG (start site) morpholino (D).

• Supplemental Figure 3 -

  Fig. S3. Injection of pcdh15b morpholinos (40 ng) does not affect hair-cell transduction. Live larvae (4 dpf) were labeled with 167 mm FM1-43 dye for 20 seconds in E3 medium. Labeled head neuromasts are shown with the focus on a neuromast near the eye (arrows). Image acquisition settings were the same for all images. No difference in uptake of FM1-43 was observed among uninjected (A), 4 bp mismatch control (B), pcdh15b GT (splice site) (C) or pcdh15b ATG (start site) morpholino-injected larvae (D). Scale bar: 20 mm.