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Fig. 1. Zebrafish have two pcdh15 genes. (A) Cloning of pcdh15a.
The orbiterth236b locus maps to chromosome (LG) 13 between
the simple sequence length polymorphic (SSLP) markers z26404 and z25253. The
critical interval was determined by generating SSLP markers from overlapping
BAC and PAC clones and testing for recombination events in larvae identified
with the south marker z26404 (indicated by arrowheads pointing to the right)
or north marker z25253 (arrowheads to the left). Sequencing of six clones
spanning the contig identified exons of the gene pcdh15a. No other
gene was present. (B) Schematic diagram of the Pcdh15a protein. The mutations
identified in orbiter are indicated below. (C) Comparison of the
predicted extracellular and intracellular domains of zebrafish Pcdh15a and
Pcdh15b with the human and mouse PCDH15 proteins. Similarity of the
extracellular regions is in the upper right half of the table; the
intracellular regions are in the lower left half, shaded in grey.
Extracellular domains of human, mouse, and zebrafish Pcdh15 are more conserved
than the intracellular domain. (D) Phylogeny of human, mouse, and zebrafish
Pcdh15 proteins are shown. The scale indicates phylogenetic distance by
substitution events.
