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Fig. 4. orbiter mutants have a splayed hair-bundle phenotype. (A-D) The
actin of the mechanosensory hair bundles of the lateral cristae were labeled
with Alexa488-Phalloidin, and visualized in transverse sections with a
confocal microscope. In wild-type larvae (A), the bundles are conical and
intact in comparison to bundles in orbiterth263b (B). A
similar phenotype is visible tc256e bundles (C), but many intact
bundles are still present. A null allele of mariner/myosin VIIA (D)
causes a more severe phenotype than th263b (B). (E,F) Horizontal
optical sections of lateral line hair bundles. The unstained insertion point
of the kinocilium is located at the caudal or rostral margin of the hair
cells. No differences in planar polarity were observed between wild-type or
th263b bundles as indicated with arrows (pointed toward each
kinocilium) in (E') and (F'). Scale bar: 1 µm.
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