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Fig. 6. Knock-down of pcdh15b function shows a dose-dependent effect on
photoreceptor structure. (A-I) Cryosections of the proximal region of day 4
retinas were stained with Alexa568-Phalloidin (red), and DAPI (blue). All
layers are present in the ATG Mo-injected larvae (B,C). The outer segments of
rod photoreceptors were stained with zpr-1 antibody (A-F, green) or double
cone photoreceptors were stained with rhod-1 antibody (G-I, green). Uninjected
larvae (A,D,G), larvae injected with 30 ng ATG Mo (B,E,H), and larvae injected
with 40 ng ATG Mo (C,F,I) are shown. Double cone receptors are disorganized
and broader in appearance (E,F), and rod outer segments appear shorter and
clumped together (H,I). Scale bar in I, 20 µm for A-C; and 5 µm for D-I.
gc, ganglion cells; hc, horizontal cells; inl, inner nuclear layer; is, inner
segments; os, outer segments; pr, photoreceptors; rpe, retinal pigment
epithelium.
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