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Fig. 6. Cos2 is epistatic to shh and smo. (A) Eng expression in
the MPs (long arrows) and MFFs (short arrow) revealed by histochemistry in a
24 hpf wild-type embryo. (B) Eng expression is absent from the myotome of a 24
hpf shh mutant embryo. (C) Restoration of Eng-expressing MP cells
(arrows) in the myotome of a shh mutant embryo injected with
cos2 MOs (n=9/12). (D) Myotome of a 24 hpf wild-type embryo
stained with antibodies to slow MyHC showing the pattern of the SSFs. (E) Slow
MyHC immunoreactivity is completely absent from the myotome of a 24 hpf
smu mutant embryo, consistent with the lack all SSFs as well as MPs.
Like syu embryos depicted in B, smu mutants also lack all
Eng expression from the myotome (data not shown) (see also
Barresi et al., 2000;
Wolff et al., 2003). (F) Slow
fibres are restored in significant numbers in smu mutants injected
with cos2 MOs (n=15/21).
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