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Fig. 2. Genomic DNA polymorphism analysis. (A) Schematic representation indicating
the distinct imprinted genes examined. The filled boxes represent exons. The
open boxes represent the position of differentially methylated regions as
examined in this study. The lines under the graph indicate the position of the
primers used for genomic DNA polymorphism analysis. The arrows indicate the
position of the genomic polymorphisms. (B) Genomic DNA from F2 hybrids was
amplified at specific regions using primers presented in
Table 1B. PCR products were
digested with BglI for H19, DdeI for Igf2, BglII
for Igf2r, NheI for Meg9, and TfiI for
Cdkn1c. Parent-of-origin alleles are distinguished by the size of the
DNA fragments generated by digestion and visualized by ethidium bromide stain
after agarose gel electrophoresis. Mice that were homozygous for 129 alleles
and homozygous for Czech alleles served as controls. Only embryo #2 but not
embryo #1 showed appropriate polymorphisms at the maternally expressed
H19, Igf2r, Meg9, and Cdkn1c genes and at the paternally
expressed Igf2 gene.
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