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Fig. 6. Binding of Lsh to the DMR of Cdkn1c. Chromatin immunoprecipitation
assays were performed in 3T3 cells that had stably integrated the Flag-Lsh
expression plamids. Specific antibodies directed against the Flag-tagged form
of Lsh and murine control antibodies of the same isotype were used. Aliquots
of chromatin taken before immunoprecipitation were used as `Input' controls.
The immunoprecipitates obtained were PCR amplified using the primers specific
to the DMRs of indicated genes.
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