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Fig. 3. Expression of regulatory genes expressed in the MFP of wild-type embryos is not maintained in mol-/- mutants. Dorsal (A-E,J,K) and lateral (F-I) views of wild-type (D,F,H,J) and mol-/- embryos (E,G,I,K), at ages shown in the bottom left-hand corner, analysed for expression of various genes (indicated in the bottom right-hand corner). (A-C) Clutches of embryos from parents heterozygous for the moltv53a mutation. No obvious differences in twhh, her9 or ntn1 expression in prospective floorplate between wild-type and mol-/- mutants is evident at these early stages. The arrowheads in D,E show gaps in floorplate shh expression in the mol-/- embryo compared with wild type.





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