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Fig. 2. Blue bars, total percentage of 5BrdU-labeled cells; pink bars, percentage of 3HTdR label-retaining cells; green bars indicate the percentage of double label (3HTdR/5BrdU)-containing nuclei. After the two day 5BrdU pulse (post-pulse), 7.3±0.9% of the nuclei were 5BrdU-positive including ~1.8% of the total number of 3HTdR label-retaining cells (82.8±0.9% of LREC). Following a 5 day chase (post chase), the percentage of 5BrdU-positive nuclei increased to nearly 12%, while the percent of doubly labeled 3HTdR/5BrdU nuclei dropped to 0.6%(14.7±3.0% of LREC) and the singly labeled 3HTdR-retaining cells rose to roughly 1.6% (85.2±3.0% of LREC) of the total nuclei counted. *The percent of LREC in the population after 5BrdU incorporation did not vary significantly, remaining near 2.0%.





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