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Fig. 1. Spatiotemporal expression profiles of Brn3a and Brn3b in developing mouse retinas. (A-L) The expression of Brn3a and Brn3b in RGCs. (A) The onset of Brn3b expression (green) starts at E11.5 in the central retina. (D,G,J) At E12.5 to E15.5, Brn3b expression expands towards the peripheral retina and is detected in cells in the NBL and in post-migration RGCs in the GCL. (B) Brn3a expression (red) is not detected in the retina at E11.5. (E,H,K) The appearance of Brn3a begins at E12.5 and is limited to RGCs in the GCL throughout the development. (C,F,I,L) Overlaid images of Brn3a and Brn3b expression. (M-R) Postmitotic expression of Brn3b in the developing retina. (M,P) Anti-Brn3b labeling shows Brn3b in nuclei of cells in the GCL and the NBL. (N) Anti-BrdU (red) labels the nuclei of proliferating cells at S phase. (O) Merged image of M,N. (Q) Anti-PH3 (green) reveals the nuclei of proliferating cells at M phase. (R) Merged image of P,Q. Inserts showed the enlarged view of the corresponding boxed regions. NBL, neuroblast layer; GCL, ganglion cell layer; l, lens. Scale bar: 100 µm.





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