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Fig. 3. Persistent projection neurons prune processes locally during early
metamorphosis. (A-D) Timecourse for PPN dendritic pruning at the larval AL.
(A) At the onset of puparium formation, each PPN labeled using GH146 MARCM has
larval morphology, with dense dendrites in one glomerulus of the larval AL
(score of 1). (B) As metamorphosis proceeds, dendrites in the larval AL start
to disappear (score of 2). (C) Soon, only a few dendritic remnants are visible
in the larval AL (score of 3). (D) Eventually, all dendritic remnants vanish
from the larval AL (score of 4). (E-H) Timecourse for PPN axonal pruning at
the larval MB and LH. (E) At puparium formation, each PPN has an axon with one
or two large synaptic densities in MB calyx and branches in LH (score of 1).
(F) The synaptic bouton in MB calyx shrinks as branches in the LH disappear
(score of 2). (G) Soon only a slight swelling in the MB calyx indicates the
former bouton and the main axon branch has begun to fragment (score of 3). (H)
At last, no trace of the bouton(s) in MB calyx can be seen and the axon has
pruned back to the edge of the MB calyx (score of 4). (I-K) Quantification of
pruning scores of PPN dendrites in the (I) larval AL, (J) MB calyx and (K) LH
every 2 hours APF. 0 hour APF brains were stained with anti-mCD8 and nc82, all
others with anti-mCD8 and anti-synaptotagmin. Slender arrows indicate
dendrites in the larval AL. Arrowheads indicate synaptic boutons in the MB
calyx glomeruli. Blunt arrows indicate axon termini in the LH.
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