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Fig. 1. ceh-16 structure, mutants and expression pattern. (A) Exon/intron
structure of ceh-16 (C13G5.1) on chromosome III. The ceh-16
gene is organized in five exons, the start methionine being in the second exon
and the stop codon in the fifth exon. Structure of the deletion mutants.
Bottom: structure of translational gfp fusion constructs used in this
study. In both constructs ceh-16 expression is driven by the
endogenous promoter region and contain the unc-54 3' UTR of the
plasmid pPD95.75. The construct on top rescued the ceh-16 phenotype
mutant. (B) Amino acid sequence of the proposed ceh-16 gene product
(187 aa), underlined: epitope for Mab 4D9
(Patel et al., 1989). (C,D)
Expression pattern of the rescuing gfp construct is most robust from
250 minutes after the first cleavage (C) throughout embryonic development (D)
(1.5-fold stage of elongation) until early 3-fold stage (not shown).
Expression was observed in the nuclei of hyp5, H0-H2, V1-V6, T. Some of these
cells are not in the focal plane of the pictures. (E,F) Antibody staining of
embryos at the same developmental stages as shown in C,D. All nuclei that
expressed ceh-16::gfp were also stained with the monoclonal antibody
4D9 (Patel et al., 1989).
(G,H) Schematic representation of the position of ceh-16 expressing
cells [based on Sulston et al. (Sulston et
al., 1983)]. Scale bar: 10 µm.
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