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Fig. 4. ceh-16 regulates early seam cell markers. (A,C,E) Wild-type
expression by means of integrated gfp constructs [elt-5
(Koh and Rothman, 2001)] or
extrachromosomal arrays [nhr-73 and nhr-74
(Miyabayashi et al., 1999)].
(B,D,F) corresponding Nomarski micrographs. (G-L) Corresponding gfp
strains in which ceh-16 was knocked down by ceh-16(RNAi).
(K,L) Dorsal view. All the markers were downregulated and all animals show the
phenotypic hallmarks of ceh-16(—), although to a lesser extent
due to lower penetrance in RNAi experiments
(Table 1). (M-P)
ceh-16 ectopically induces elt-5::gfp expression. Ectopic
expression of ceh-16 is achieved by a heatshock-inducible promoter
(see Materials and methods). (M) Effects of ceh-16 misexpression in
the epidermis; upper arrow points to irregularities at the tip of the tail,
which are reminiscent of failed fusion events [similar as in eff-1
mutants (Mohler et al.,
2002)]. (N) Ectopic expression of elt-5::gfp (arrows).
(O) Similar to M (upper rightmost arrow points to the anus). (P) Lower arrow
(also entire lower margin of the larva) points to ectopic dorsal expression of
elt-5::gfp. Scale bars: 10 µm for all embryos; 20 µm for all L1
larvae.
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