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Fig. 1. Expression profiles of GATA4, 5 and 6 and their differential induction of endodermal markers in cap explants. Xenopus embryos were collected at a range of early developmental stages and total RNA was extracted for real-time RT-PCR, monitoring Sox17 ${alpha}$ (A) and GATA4-6 (B). (C) In situ hybridisation on serial sections of stages 10, 10.5 and 11 embryos, using GATA4 and 6 as probes. Arrowheads mark the dorsal blastopore lip. (D) HA-tagged Xenopus GATA4-6 synthesised in animal caps detected with rat monoclonal anti-HA antibody and anti-rat polyclonal antibody conjugated to peroxidase. LG6: long GATA6, SG6: short GATA6. G4 and G5: GATA4 and 5. Erk2 is a MAPK detected using rabbit polyclonal IgG and anti-rabbit antibody conjugated to horseradish peroxidase, as a loading control. (E,F) Induction of Sox17 ${alpha}$ , HNF1ß (E) and HNF3b (F) revealed by real-time RT-PCR. Error bars represent standard deviations from the mean of three measurements of the same cDNA. Each experiment was repeated three times.

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