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Fig. 5. Skeletal phenotypes of MFCS1 and Shh KO mutants. (A-C) Skeletal preparations of E14.5 embryos were stained with alcian blue. (A,B) Lateral view of a wild-type and MFCS1 KO homozygote. (C) Dorsal view of a Shh KO homozygote. (D-I) Skeletal preparations of 3-week-old mice were stained with alizarin red and alcian blue. The forelimb and hindlimb of the wild-type mouse are shown in (F) and (I) respectively. (D,E) Forelimbs of MFCS1 KO homozygotes. (G,H) Hindlimbs of MFCS1 KO homozygotes. cl, clavicle; eb, elbow joint; fb, floating skeletal element; fe, femur; fi, fibula; h, humerus; mc, metacarpals; mt; metatarsal bones; ph, phalanges; r, radius; s, scapla; sp, stylopod; t, tibia; ts, tarsal bones; u, ulna; zg, zeugopod; zv, zeugopodial vestiges.





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