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Fig. 3. Lfng–/– females ovulate in response to
exogenous hormones, but oocytes are not at meiotic metaphase II. (A) After
hormone administration, OCC were collected from the oviduct. Oocytes were
stained with anti-
-tubulin-FITC and Hoechst 33258, and visualized using
confocal microscopy at 800 x final magnification. Genotypes are as
indicated. In Lfng+/+ and
Lfng+/– oocytes, note the presence of the polar
bodies (white arrowhead) and chromosomes aligned on a metaphase spindle. Many
Lfng–/– oocytes contained one or more bodies
with scattered chromatin (white arrowheads). Some
Lfng–/– oocytes were at anaphase/telophase I;
note the lack of a polar body. (B) Representative kinase assays analyzed on
15% SDS-PAGE and visualized using a phosphorimager. Lanes are as marked. MBP,
myelin basic protein. (C) Lfng+/– and
Lfng–/– mice were induced to ovulate and OCC
were collected from the oviducts. OCC demonstrated normal cumulus expansion in
Lfng null mutant females, under light microscopy at 70 x
magnification. (D) Graphs show relative kinase activity of MPF and CSF, the
kinase assays were quantified and expressed as fold activity over control
(n=9/genotype) ±s.d.
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