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Fig. 5. Expression of Notch family genes in the
Lfng–/– ovary by ISH. Gene-specific
digoxigenin labeled probes were used, as indicated; representative data from a
minimum of three replicates of Lfng+/– and
Lfng–/– are presented. Notch3 demonstrated no
change in follicle stage or cell-type-specific expression in null ovaries;
black arrows indicate granulosa cell staining and the red arrowhead indicates
expression in an early CL. Jagged1 was limited to oocytes of small growing
follicles in the Lfng–/– ovary (arrowhead).
Expression of Notch downstream target genes Hes5, Hes1, Hesr1 and
Hesr2 was not detected in the Lfng–/–
follicles (only representative family members Hes5 and Hesr2
are presented). Note positive follicles indicated by arrows in +/– and
compare with similarly sized follicles indicated by arrowheads in
–/–.
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