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Fig. 4. Interspecies Ath5 sequence analysis identifies a highly conserved proximal non-coding region. (A) Pairwise VISTA analysis of X. laevis and mouse Ath5 5' noncoding sequences identifies a highly conserved region (black arrowhead) within the proximal region of Xath5 5' sequence, as well as a weaker region of conservation more distally (–1597 to –1669). Basepair position relative to the Xath5 translation start site (+1 bp) indicated at bottom of the VISTA alignment. Approximate location of Ath5 exon indicated by double arrowed line. (B) Alignment of the conserved region from X. laevis, X. tropicalis, mouse, human, chick, zebrafish and fugu shows blocks of highly conserved nucleotides (gray). Bars mark two conserved E-box binding sites (CANNTG) found in all species.





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