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Fig. 6. 3.3 kb Xath5 transgene expression can be activated by bHLH factors, but is not dependent upon conserved E-boxes or bHLH activity. (A-C) Injection of RNA encoding RFP alone did not alter transgene expression on the injected side. (D-E) Injection of RNA for Xath5 ectopically activated transgene expression on the injected side (bracket in E) compared with the uninjected side (D). RFP marks the region targeted on the injected side (bracket in F). (G) Mutation of E1 and E2 ({Delta}E1,2) in the pG1X5-3.3 kb transgene did not eliminate retinal transgene expression. Mutation of two additional conserved E-boxes, E3 and E4, also did not abolish transgene activity. (H-J) Injection of RNA for the dominant-negative Xath5-EnR did not suppress expression of the pG1X5-3.3 kb transgene on the injected side (I) compared with the uninjected side (H). RNA encoding RFP (red) was co-injected to mark the injected side (J).





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