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Fig. 5. PFS2 transcripts were present in developing primordia. The
localization of PFS2 transcripts was determined by in situ
hybridization. (A) Transcripts were present in the suspensor (su), developing
embryos (e), and (B) endosperm (en). Note that the brown color in the
endothelium (et) derives from a naturally occurring pigment in these cells.
(C) In seedlings, transcripts are present in the shoot apical meristem (sam)
and leaf primordia (lp), but absent in mature cotyledons (c) and leaves. (D)
Similarly, this gene is expressed in floral meristems (fm) and floral
primordia (fp). (E) In developing flowers, PFS2 mRNA was most
prevalent in the developing anthers (a) and ovule primordia (op). (F,G) In
ovules the transcripts localized to the chalaza (ch) and nucellus (n). (H)
Extended incubation of slides in substrate reveals that PFS2 is also
expressed in carpel walls and petals (p). (I) The anti-sense probe control did
not stain. (J) The relative abundance of PFS2 transcripts was
measured using RT-PCR and expressed as a fraction of the level found in
wild-type inflorescences. RNA for cDNA synthesis was extracted from: (1)
wild-type pistils; (2) the outer three whorls (sepals, petals, and stamens);
(3) mature leaves; (4) wild-type inflorescences; (5) pfs2 mutant
inflorescences. GAPC transcripts were measured to determine if equal
amounts of template were used in each PCR reaction. Scale bars in A,B,F: 20
µm in A,B,F; 50 µm in C-E,G-H. f, funiculus; g, gynoecium; s, sepals;
tt, transmitting tract.
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