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Fig. 7. PFS2 activity repressed AG expression. (A) In ag mutant
flowers, repeating whorls of sepals and petals develop. In addition, the loss
of floral determinacy in ag mutants results in many more floral
whorls than the four found in wild-type flowers. (B) Except for undulating
petal edges, flowers in the ag pfs2 double mutant appeared similar to
ag single mutants. (C) Petals in pfs2 mutants curled
downward. (D) In ag pfs2 mutants, the leaf curling phenotype was
attenuated, but not eliminated. (E) RNA was extracted from flowers and leaves
of pfs2 mutants, wild-type plants, and PFS2 OE plants.
Relative AG mRNA levels were measured by RT-PCR and expressed as a
fraction of the level found in pfs2 mutants. As an internal control,
GAP mRNAs were simultaneously amplified. The OE1 and OE2 plants
exhibited phenotypes similar to that shown in
Fig. 6C and
Fig. 6B, respectively. (F) In
wild-type flowers, AG transcripts were present in developing floral
primordia (fp), anthers (a) and gynoecia (g). (G) In pfs2 mutants,
the expression of AG in floral primordia decreased, but appeared
similar to wild type in developing anthers and gynoecia. (H) In pfs2
mutants, AG transcripts were expressed through the nucellus (n). (I)
In some of the pfs2 floral primordia, AG transcripts were
present in not only the gynoecium and stamen (st) primordia, but also in the
petal primordia (arrow). Scale bars: 50 µm in F-I. ch, chalaza; ov,
ovule.
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