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Fig. 3. RhoGEF2 and nullo/sry-{alpha} cooperate in furrow canal formation. (A-E) Embryos from RhoGEF21.1 germline clones were injected at the posterior pole with the following dsRNA: (A) Bsg25D, (B,E) nullo, (C,D) sry-{alpha}, and stained for DNA (blue), F-actin (green), Sry-{alpha} (red). Loss of Sry-{alpha} protein and loss of furrow canals (marked by F-actin, optical transversal sections, D,E) correlates with the posterior site of dsRNA injection as shown by respective antibody staining (C). Scale bars: (A-C) 50 µm, (D,E) 10 µm. Posterior to the right, arrow indicates injection site.





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