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Fig. 4. RhoGEF2 is enriched at the furrow canal. Embryos (heatfixed) stained with RhoGEF2 antibody (green, white), Dlg (red) and DNA (blue). Right half of A-D shows only the RhoGEF2 staining. (A-C,E,F) Wild type, (D) from RhoGEF204291 germline clone. (E) Surface view; wild type. (F) Cross section of an embryo undergoing ventral furrow formation. Scale bar: (A-E) 10 µm, (F) 20 µm. (G) Western blot for RhoGEF2 or Dlg of extracts from wild-type embryos and embryos from RhoGEF204291 germline clones.





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